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SEC-MALS
Types of Techniques
- Liquid Chromatography Mass Spectrometry (LC-MS)
- Gas Chromatography-Mass Spectrometry (GC-MS)
- High-Performance Liquid Chromatography (HPLC)
- Inductively Coupled Plasma Mass Spectrometry (ICP-MS)
- Combustion Ion Chromatography (CIC)
- Ion Chromatography
- Gel Permeation Chromatography (GPC) / Size Exclusion Chromatography (SEC)
- Multi-Column Chromatography (MCC)
- LC-Orbitrap
- Accelerator Mass Spectrometry (AMS)
- Nano scale Secondary Ion Mass Spectroscopy
- Secondary-ion mass spectrometry (SIMS)
- SEC-MALS
SEC-MALS
Size Exclusion Chromatography (SEC) combined with Multi-Angle Light Scattering (MALS) is a cutting-edge analytical technique used to determine the molecular weight and size distribution of macromolecules, such as proteins, polymers, and nanoparticles. This method provides precise and detailed information about the molecular characteristics of your samples.
SEC (Size Exclusion Chromatography): Separates molecules based on their size by passing them through a column filled with porous beads. Larger molecules elute first as they cannot enter the pores, while smaller molecules elute later.
MALS (Multi-Angle Light Scattering): Measures the intensity of light scattered by the molecules at multiple angles, allowing for accurate determination of molecular weight and size without reliance on molecular standards.
SEC with MALS combines the size separation capability of SEC with the precise molecular weight determination of MALS to provide comprehensive characterization of macromolecules. This technique is invaluable in research, development, and quality control processes across a wide range of industries, offering detailed insights into molecular properties without the need for calibration standards.
- Biopharmaceuticals: Characterization of proteins, antibodies, and other biopharmaceuticals to ensure purity and quality.
- Polymers: Analysis of polymer molecular weight and size distribution for material science and industrial applications.
- Nanoparticles: Measurement of nanoparticle size and distribution in nanotechnology research.
- Quality Control: Ensuring consistency and quality of products in various industries.
- Accurate Molecular Weight Determination: Provides absolute molecular weight measurements without the need for calibration standards.
- Detailed Size Distribution: Offers insights into the size distribution and polydispersity of samples.
- Non-Destructive Analysis: Samples remain intact, allowing for further analysis or use.
samples should be highly pure and filtered to remove particulates. Optimal concentration ranges from 0.1 mg/mL to 5 mg/mL, with injection volumes typically between 20 µL to 200 µL. Use compatible solvents and ensure samples are fully dissolved and stored appropriately for accurate molecular weight determination and size distribution analysis.