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Multi-column chromatography
Types of Techniques
- Liquid Chromatography Mass Spectrometry (LC-MS)
- Gas Chromatography-Mass Spectrometry (GC-MS)
- High-Performance Liquid Chromatography (HPLC)
- Inductively Coupled Plasma Mass Spectrometry (ICP-MS)
- Combustion Ion Chromatography (CIC)
- Ion Chromatography
- Gel Permeation Chromatography (GPC) / Size Exclusion Chromatography (SEC)
- Multi-Column Chromatography (MCC)
- LC-Orbitrap
- Accelerator Mass Spectrometry (AMS)
- Nano scale Secondary Ion Mass Spectroscopy
- Secondary-ion mass spectrometry (SIMS)
- SEC-MALS
Multi-column chromatography (MCC)
Multi-column chromatography (MCC), also known as simulated moving bed (SMB) chromatography, is an innovative downstream processing technique for the purification of biomolecules, pharmaceuticals, and other high-value compounds. It is a continuous chromatographic process that offers higher productivity, reduced resin consumption, and a smaller footprint compared to traditional batch chromatography.
MCC operates by utilizing multiple small chromatography columns connected in a series, with the feed, desorbent, and buffer streams continuously cycled through the system in a countercurrent manner. This countercurrent flow creates an infinite simulated column length, allowing for efficient utilization of the solid and liquid phases. The process is divided into four distinct zones: feed, desorbent, buffer, and raffinate. As the columns switch positions periodically, each zone moves in the opposite direction to the liquid flow, simulating a countercurrent movement of the solid phase. This principle enables higher loading capacities, faster flow rates, and simultaneous execution of multiple chromatographic steps, resulting in increased productivity and efficiency.
- Purification of monoclonal antibodies
- Separation of therapeutic proteins
- Isolation of peptides and oligonucleotides
- Chiral separations
- Purification of enzymes
- Separation of amino acids
- Isolation of vitamins
- Purification of vaccines
- Purification of active pharmaceutical ingredients (APIs)
- Separation of specialty chemicals
- Isolation of natural products
- Purification of food additives
- Separation of flavors and fragrances
- Isolation of nutraceuticals
- Higher productivity and throughput
- Reduced resin consumption and cost savings
- Smaller footprint and lower buffer consumption
- Continuous processing for streamlined manufacturing
- Scalable from development to production
- a) The silica and a suitable solvent
- b) solid samples – suspended in an appropriate solvent or buffer.