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Liquid Chromatography Mass Spectrometry
Types of Techniques
- Liquid Chromatography Mass Spectrometry (LC-MS)
- Gas Chromatography-Mass Spectrometry (GC-MS)
- High-Performance Liquid Chromatography (HPLC)
- Inductively Coupled Plasma Mass Spectrometry (ICP-MS)
- Combustion Ion Chromatography (CIC)
- Ion Chromatography
- Gel Permeation Chromatography (GPC) / Size Exclusion Chromatography (SEC)
- Multi-Column Chromatography (MCC)
- LC-Orbitrap
- Accelerator Mass Spectrometry (AMS)
- Nano scale Secondary Ion Mass Spectroscopy
- Secondary-ion mass spectrometry (SIMS)
- SEC-MALS
Liquid Chromatography Mass Spectrometry (LC-MS)
Liquid Chromatography–Mass Spectrometry (LCMS) is an analytical technique that marries the physical separation capabilities of liquid chromatography with the mass analysis prowess of mass spectrometry. This hybrid method has become a cornerstone in analytical laboratories due to its high sensitivity, specificity, and versatility in analysing a wide array of compounds in complex mixtures. LC-MS is a transformative technology that has significantly advanced the field of analytical chemistry. It allows for the separation, identification and quantification of chemically diverse molecules within biological, environmental and industrial samples.
The LCMS process begins with liquid chromatography, where a sample mixture is passed through a column packed with chromatographic material. Compounds in the sample interact differently with this material, causing them to elute at different times, known as retention times. These separated compounds are then introduced into a mass spectrometer, where they are ionized, and their mass-to-charge ratios are measured. The resulting mass spectra provide characteristic fingerprints for each compound, facilitating their identification and quantification.
LCMS is extensively used for drug development, pharmacokinetics and toxicology studies. It aids in the identification of drug metabolites and the quantification of drugs within biological fluids.
Environmental Analysis: (Pollutant Analysis)LCMS helps detect and quantify pollutants and toxins in environmental samples, contributing to environmental protection and regulation compliance.
Food Safety:The technique is employed to ensure the safety and integrity of food products by detecting contaminants like pesticides and antibiotics.
Clinical Diagnostics:LCMS supports clinical diagnostics by measuring biomarkers and metabolites, providing insights into disease states and therapeutic drug monitoring.
Forensic science:In forensics, LC-MS helps analyse biological evidence like blood, urine, and tissue samples. This can be used to identify drugs, toxins and other compounds that may be relevant to a criminal investigation.
- Highly Sensitive
- Excellent Specificity
- Broad Application
- Structural Information
(Dissolve solid samples in a suitable solvent like water, methanol, acetonitrile, or a combination thereof.)
Quantity:Solid Sample – 2 gm. Liquid Sample – 2 ml.